Effects of Dietary Bitter Melon Extract on Growth Performance, Antioxidant Capacity, Inflammatory Cytokines Expression, and Intestinal Microbiota in Common Carp (Cyprinus carpio L.)

Table 2

Primer sequences for RT-qPCR detection of inflammatory cytokines genes.

Gene	Primer	Sequence(5-3)	Accession number	Product size	Amplification efficiency
il-1β	F	TTACAGTAAGACCAGCCTGA	AJ245635	91 bp	96%
	R	AGGCTCGTCACTTAGTTTGT
il-6	F	GCAGCGCATCTTGAGTGTTTAC	AY102632	73 bp	97%
	R	CTGCTGCTCCATCACTGTCTTC
il-8	F	GTCTTAGAGGACTGGGTGTA	EU011243.1	110 bp	98%
	R	ACAGTGTGAGCTTGGAGGGA
il-10	F	CGCCAGCATAAAGAACTCGT	KX964678.1	233 bp	96%
	R	TGCCAAATACTGCTCGATGT
18S	F	CTGAGAAACGGCTACCACATC	FJ710827.1	107 bp	95%
	R	GCCTCGAAAGAGACCTGTATTG