Effects of Dietary Carbohydrate Levels on the Growth and Glucose Metabolism of Juvenile Swimming Crab, Portunus trituberculatus

Table 2

Real-time PCR primer sequences for analysis of gene expression in hepatopancreas of swimming crab.

Gene names	Primers		Access no.
	Forward (5-3)	Reverse (5–3)
gk	AGGTGGACCAACACTCTCGC	TCCAGCAAGCCACAGGTCTC	XP_023223115
pk	ACACCAAAGGACCCGAAATC	ATGCCGCCATTCTCTACCTC	ALK82311
hk	TATGTGGCAGGAATCGTGTC	GGAGTCTATCAGCAATGGCG	ABO21409
g6pase	ATGTGGACGCTGCTCTTCTG	CACCATCCAAGTGGCATACC	ALK82315
fbpase	CAGAAGAAAATCCCTCACGC	CCACATACTTCCCCTGACGA	AMJ52089
pepck	CGAGCCGCTACCCAAATA	CGAAGTCGTCCTCGTTGA	AAL78163
glut1	TGGTGCGGAACTCCAATCTA	GAAGCCTATGCCGACAATGA	AIT97017
glut2	CGATGGGAGCCTTGAGTTTT	ACAGGATTCCAACCACGACC	ALG65274
gsk	AGGTGCTCCAGGATAAACGG	ACACACCCAGCGAGTGAATG	ASW35107
foxo	CATAAGTTCTCGCCAGCCTC	TAACCTTCAGGACACGGGAG	XP_023216972
igf1r	CACTCACCAGGAGCCCATCTA	TCAGAGAGTTTCACAACCCGC	XP_023716772
pi3k	AGCCACCACTCGCTGAACA	GGGATGGGACTCTGCTGAAG	ADE44090
fas	CTTCAATACCCACCAAACC	CCTCAATGATGCCAGACAC	Sun et al. [72]
acc	TCTCAGGGCAACCTTACGCT	CGGGAGGCAGTAACCATTCA	Sun et al. [72]
srebp1	GTGATGTGTGCCTTGCGAGT	CCAGGGTTCACCAGTGTAGT	Sun et al. [72]
6pgd	GGGTGGAACCTCAACTATGG	CGATAGCCATCATAGAAAGCC	Sun et al. [72]
g6pd	TGAAAAGGTGAAGGTGCTGA	CGGTGGAGTCATCAAGGTAAC	Sun et al. [72]
cpt1	GCTTGCCTACTACCGACAC	CCTTGGACATCTTACTGCTC	Sun et al. [72]
cpt2	TGGGACAAGGTTTTGATAGGC	TGGAGATGATGATGTGGTTGA	Sun et al. [72]
tor	TGTGGACATAGGGCAAACTG	GACCGCTTCACCAAATCATC	Zhan et al. [18]
s6k1	CGCCCCTCAGATTTCCAGT	TCTCAGCCTTTGTGTGCG	Zhan et al. [18]
akt	GGACTACGAGGCACCAAGAA	TGGACCACTTCATCACGCTC	Zhan et al. [18]
β-actin	GAAGTAGCCGCCCTGGTTGT	GAATACCTCGCTTGCTCTGC	Sun et al. [72]