Regulation of Hypoxia-Induced Cell Death in Human Tenocytes

Figure 2

Hypoxia rapidly induces HIF-1α expression in human tenocytes. Primary human tenocytes were cultured in DMEM/F12 culture medium containing 10% FCS under normoxic conditions until the cells reached 70% confluence. The cells were exposed to total hypoxia (0.1% O₂) for 1, 4, 8, 16, 24, and 48 h. (a) Representative Western blot for HIF-1α in tenocytes. (b) HIF-1α expression qualified by densitometry is presented as mean ± SE. (𝑛=3). ^∗𝑃<0.05 versus normoxic control; ^∗∗𝑃<0.01 versus normoxic control; ***𝑃<0.001 versus normoxic control.

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