Nanotomography of Cell Surfaces with Evanescent Fields

Figure 4

TIRFM images recorded at Θ=66^∘ (left) and cell-substrate topology (right) of U373MG glioblastoma cells upon incubation with laurdan either (a) without further treatment or 30% cholesterol depletion (by 1 mM MβCD; (b) or (c) 50% cholesterol depletion (by 4 mM MβCD). Fluorescence excited at 391 nm and detected at 𝜆≥415 nm; image size: 210 𝜇m × 210 𝜇m).

(a)

(b)

(c)