Research Article

A Ratiometric Fluorescence Imaging System for Surgical Guidance

Figure 4

Relative PpIX fluorescence intensities as a function on position on the CCD image (centered at the 400 pixel value), showing how this varies with the angle between the optical axis (i.e., laparoscope direction) and the tissue surface, for the different optical properties: (a) 0°, (b) 15°, (c) 30°. Graphs on the left are the uncorrected data; those on the right are after applying the double-ratio algorithm (with linear fits to the combined data). In each plot, the signals are summed along the axis perpendicular to the x-axis of the graphs on the 3D CCD array. All measurements were made at 2 cm working distance.
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(a)
532368.fig.004b
(b)
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(c)