A Rapid, Isocratic HPLC Method for Determination of Insulin and Its Degradation Product

Table 1

Insulin calibration curve data.

Con. (mg/mL)	Cal. 1	Cal. 2	Cal. 3	Cal. 4	Average	SD	RSD %
	AR*	AR	AR	AR
0.6810	11.8730	11.8194	11.8894	11.9334	11.8788	0.0471	0.40
0.3405	5.8769	5.7887	5.8790	5.8062	5.8377	0.0471	0.81
0.1703	3.0726	3.0054	3.0551	3.0715	3.0512	0.0315	1.03
0.0851	1.4171	1.4513	1.4614	1.4350	1.4412	0.0194	1.35
0.0426	0.7468	0.7283	0.7324	0.7504	0.7395	0.0108	1.46
0.0213	0.3682	0.3681	0.3601	0.3688	0.3663	0.0041	1.13
0.0106	0.1872	0.1802	0.1792	0.1811	0.1819	0.0036	1.96
Slope	17.4223	17.3179	17.4471	17.4714	17.4147	0.0676	0.39
Intercept	−0.0003	−0.0088	−0.0030	−0.0091	−0.0053	0.0044
𝑅^2	0.99983	0.99985	0.99989	0.99969	0.99982	0.00009	0.01

∗AR: area ratio.