A Rapid, Isocratic HPLC Method for Determination of Insulin and Its Degradation Product

Table 2

Data of precision experiment (𝑛=6, each preparation was injected twice).

Conc.	0.06810 mg/mL					0.01703 mg/mL
	Ins. area	IS area	AR	Ave.	Conc.	Ins. area	IS area	AR	Ave.	Conc.
	9.9868	8.1734	1.2219	1.2260	0.07070	2.6968	8.8942	0.3032	0.3057	0.01786
	9.9792	8.1126	1.2301			2.7007	8.7643	0.3082
	11.7923	9.7909	1.2044	1.2004	0.06923	2.7409	9.0975	0.3013	0.3025	0.01767
	11.6048	9.7004	1.1963			2.7348	9.0055	0.3037
	11.6483	9.5581	1.2187	1.2212	0.07043	2.8628	9.7853	0.2926	0.2926	0.01711
	11.6895	9.5531	1.2236			2.8648	9.7891	0.2926
	10.9214	9.0979	1.2004	1.2117	0.06988	3.1066	10.1297	0.3067	0.3081	0.01800
	11.0028	8.9965	1.2230			3.1642	10.2212	0.3096
	11.2385	9.2503	1.2149	1.2206	0.07039	3.1953	10.6644	0.2996	0.2989	0.01747
	11.3289	9.2381	1.2263			3.1761	10.6510	0.2982
	10.8315	8.9431	1.2116	1.2003	0.06923	2.9253	9.6708	0.3025	0.3048	0.01780
	10.6340	8.9408	1.1894			2.9792	9.7013	0.3071
Average					0.06998					0.01765
SD					0.00064					0.00032
RSD %					0.91					1.82