Table of Contents
Advances in Pharmaceutics
Volume 2015, Article ID 567032, 5 pages
Research Article

Determination of Glycerin from a Marketed Personal Care Product Using Gas Chromatography

R&D Division, Dey’s Medical Stores (Mfg.) Ltd., 62 Bondel Road, Kolkata, West Bengal 700019, India

Received 20 September 2014; Revised 25 December 2014; Accepted 16 January 2015

Academic Editor: Maria Cristina Bonferoni

Copyright © 2015 Amit Kumar De et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The current study presents a packed column gas chromatographic technique for the estimation of glycerin using a flame ionization detector from a marketed hair tonic in presence of resorcinol, ethanol, biotin, keratin hydrolysate, undecylenic acid alkylolamide (hyalkyl HBU), D-biotin, nicotinic acid, and polyvinylpyrrolidone. The validation studies show the proposed method to be specific, sensitive, precise, and accurate. The method is found to be linear in the concentration range 1.25 mg/mL to 10.02 mg/mL with value 0.99. The limit of detection and the limit of quantitation were 0.01 mg/mL and 0.05 mg/mL, respectively. The method does not involve any complex sample preparation procedure and is therefore suitable for regular analysis of glycerin from marketed hair tonic.