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Advances in Physical Chemistry
Volume 2018, Article ID 4712547, 9 pages
Research Article

Enhancement of Electrochemical Performance of Bilirubin Oxidase Modified Gas Diffusion Biocathode by Porphyrin Precursor

CFD Research Corporation, 701 McMillian Way, Suite D, Huntsville, AL 35806, USA

Correspondence should be addressed to Sameer Singhal; moc.crdfc@lahgnis.reemas

Received 16 December 2017; Revised 12 March 2018; Accepted 28 March 2018; Published 3 June 2018

Academic Editor: Ramasamy Karvembu

Copyright © 2018 Erica Pinchon et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Recent studies have focused on tailoring the catalytic currents of multicopper oxidase (MCO) enzymes-based biocathodes to enhance oxygen reduction. Biocathodes modified with natural substrates specific for MCO enzymes demonstrated drastic improvement for oxygen reduction. Performance of 1-pyrenebutanoic acid, succinimidyl ester (PBSE), and 2,5-dimethyl-1-phenyl-1H-pyrrole-3-carbaldehyde (Di-Carb) oriented bilirubin oxidase (BOx) modified gas diffusion biocathode has been highly improved by incorporating hematin, a porphyrin precursor as electron transfer enhancement moiety. Hematin modified electrodes demonstrated direct electron transfer reaction of BOx exhibiting larger O2 reduction in current density in phosphate buffer solution (pH 7.0) without the need of a mediator. A remarkable improvement in the catalytic currents with 2.5-fold increase compared to non-hematin modified oriented BOx electrodes was achieved. Moreover, a mediatorless and compartmentless glucose/O2 biofuel cell based on DET-type bioelectrocatalysis via the BOx cathode and the glucose dehydrogenase (GDH) anode demonstrated peak power densities of 1 mW/cm2 at pH 7.0 with 100 mM glucose/10 mM NAD fuel. The maximum current density of 1.6 mA/cm2 and the maximum power density of 0.4 mW/cm2 were achieved at 300 mV with nonmodified BOx cathode, while 3.5 mA/cm2 and 1.1 mW/cm2 of current and power density were achieved with hematin modified cathode. The performance improved 2.4 times which attributes to the hematin acting as a natural precursor and activator for BOx activity enhancement.

1. Introduction

Biofuel cell technology has received much attention as energy harvesting devices for powering portable devices and microscale electronic systems. Enzymes functionalized with nanomaterials such as carbon nanotubes, graphene, and nanoparticles provide extremely powerful platforms for wide range of biofuel cell applications that are capable of operating independently over a prolonged period of time, without the need of external recharging or refueling of devices. Enzymes such as multicopper oxidases (MCOs) belonging to oxidoreductase family can reduce oxygen into water performing oxygen reduction reaction (ORR). These reactions have been extensively studied and described in previous literature [14]. The most common “Blue” MCO enzymes are ascorbate oxidase (AOx), laccase (Lac), and bilirubin oxidase (BOx) that can act as excellent biocathode materials for biofuel cell applications [5, 6]. These enzymes are more active and selective than the state-of-the-art electrocatalyst platinum because BOx can promote a four-electron reduction of oxygen [7, 8], leading directly to water rather than production of significant amount of hydrogen peroxide (H2O2- 2-electron exchange) [9]. It has been shown previously that BOx demonstrated high electrocatalytic oxygen reduction and low overpotential necessary to catalyze the reaction and a turnover rate of 0.7 O2 per Cu·s−1, while Pt catalysts turnover rate is three times lower at overpotential of 350 mV [2, 10].

Moreover, BOx enzymes are well known to perform ORR when immobilized onto the surface of solid supports. Enhanced activity has been observed when functionalized with nanomaterials, since nanomaterials have been considered as excellent scaffolding structures for immobilization of the enzymes without sacrificing their bioactivity. The ORR is mainly dependent on the structure of BOx which was well studied and reported previously [11, 12]. It contains three different copper centers (T1, T2, and T3) with overall four copper ions that catalyze the oxidation of bilirubin to biliverdin [13], thereby reducing molecular oxygen to water. The mechanism of electron transfer involves the T1 site of MCO acting as the primary electron acceptor from the substrate via an intramolecular electron transfer (IET) to the T2/T3 cluster site which converts molecular oxygen to water. Recent research unveiled whether the ORR in BOx is a four-electron transfer or a two-electron transfer with a hydrogen peroxide intermediate [7, 14]. Much research has also been devoted in understanding the BOx direct electrochemistry (DET) and electron transfer via mediation by redox mediators [1517]. Nevertheless, the use of these enzymes as biocatalysts has not yet been generally adopted for commercial purposes.

One persistent challenge is maintaining the catalytic activity of the enzyme and improving the performance often when immobilized on a solid support [18, 19]. Enzymes may adsorb successfully and however tend to denature rendering some of the immobilized enzyme inactive and ineffective [2022]. To overcome these challenges, enzymes that catalyze electron transfer reactions must be entrapped in hydrogels or stabilized with the use of orienting agents. Orienting agents promote correct and proper allocation of enzymes on the surface of the electrode to obtain high current density. The lone copper on BOx should not be no more than 1-2 nm from the electrode surface to avoid interfacial electron transfer being the rate-limiting step in oxygen reduction electrocatalysis [23, 24].

Recent studies have demonstrated a trend in surface modifications at biointerface level for MCOs based biocathode that incorporates the use of aromatic, hydrophobic, and hydrophilic molecules in order to suitably orient these redox enzymes with the T1 copper site immobilized on carbon nanotube sidewalls to enhance the oxygen electroreduction [25]. These molecules are natural substrates that are specific for the MCO enzymes for oxygen reduction. Laccase was initially studied by the Armstrong group where the hydrophobic pocket of laccase interaction with polycyclic aromatic compounds such as anthracene resulted in remarkable enhancement of electrocatalytic currents. The aromatic compounds structure is very similar to the natural substrate of Lac (phenols), and the strong hydrophobic interactions have been reported to promote the apt orientation for the DET [26]. Similar strategy was extended to the BOx enzymes where the studies show that the substrate-pocket did not exhibit hydrophobic interactions but electrostatic interactions, which are an efficient way to achieve direct wiring of BOx [27]. Along this line, different literature reports have focused on incorporating specific substrates of BOx such as bilirubin [28], quinones [29], and syringaldazine [30] towards appropriate orientation that can be convenient for DET-type electrocatalysis. Our group has previously reported on crosslinking the enzyme to the electrode with orienting agents, two bilirubin functional analogues, pyrrole-2-carboxaldehyde and 2,5- dimethyl-1-phenyl-1H-pyrrole-3-carbaldehyde, for enzyme orientation and 1-pyrenebutanoic acid, succinimidyl ester (PBSE) as the tethering agent. These compounds were chosen because they each contain a pyrrole moiety functionalized with a carbonyl group. Thus, the electronegative N-atom from the pyrrole moiety and the O-atom from the aldehyde group can act as hydrogen bond acceptors and the H-atom as a hydrogen bond donor [29]. Subsequently, we reported on the utilization of syringaldazine (Syr), for enzyme orientation, of both Laccase and BOx that demonstrated approximately 6 and 9 times increase in current density, respectively, compared to physically adsorbed and randomly oriented Lac cathodes [30].

Our present study follows from observations that the activity and the performance of the oriented BOx were further enhanced from incorporating a porphyrin precursor solution—hematin—on the biocathode. Hematin is a ferriprotoporphyrin-IX with a hydroxide ion bound to the ferric ion formed when hemin (ferriprotoporphyrin-IX with a chloride ion bound to ferric ion) is treated with strong NaOH solution. Hemin is an active center of family of hemeprotein, such as b-type cytochromes, peroxidase, myoglobin, and hemoglobin. The first electrochemical behavior of hemin was studied in 1968 on a platinum electrode by coulostatic method [31]. Hemin adsorption on graphite electrode demonstrated fast electron transfer that can exceed monolayer coverage with high amount of active species. Several literature studies show that hemin modified electrodes were extended in the catalysis and reduction of hydrogen peroxide [3234], oxygen [35], and superoxide [31]. Utilization of hemin, protoporphyrin derivatives as pretreatment of the surface for improving the activity of BOx on cathode electrode has also been reported [36].

Herein, we demonstrate the enhancement of BOx air breathing cathode performance via the modification of the electrode with hematin. We also investigated the capability and effects of hematin versus hemin to promote an efficient electron transfer mechanisms for oxygen reduction. We further constructed a mediatorless and compartmentless glucose/O2 DET-type biofuel cell to investigate the cell performance.

2. Materials and Methodology

Hemin, 1-pyrenebutanoic acid, succinimidyl ester (PBSE) was obtained from Setareh Biotech LLC; 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) was obtained from TCI America; and N-hydroxysuccinimide ± 98% (NHS) was obtained from Alfa Aaesar. Hematin (94.5%) was obtained from Chem-Impex Int’l Inc. NAD-dependent glucose dehydrogenase (GDH, from Pseudomonas sp., E.C. and glucose were obtained from Sigma Aldrich and used as received; BOx was obtained from Myrothecium verrucaria (EC, Amano Enzyme U.S.A. 50 Co., Ltd. Multiwalled nanotubes (MWNTs) paper (Buckeye Composites), MWNTs ( = 20–30 nm, = 10–30 μm), and single walled nanotubes (SWNTs) (99% purity) were obtained from

3. Electrode Preparations

3.1. Hemin and Hematin Modified Electrodes

Air breathing cathodes were fabricated as in the previously described procedure with slight modification [37]. Briefly, teflonized carbon black powder (35% teflonization and 50% teflonization, XC35 and XC50) and MWNTs paper (Buckeye Composites) were hydraulically pressed for 5 minutes at 500 psi. Figure 1 shows the schematic illustration of fabrication of gas diffusion layer (GDL) cathode (a) and image of the biofuel cell (b). A 10 mM hemin solution was prepared by mixing hemin into DMSO. A 10 mM hematin solution was prepared by mixing hemin into 20 mM sodium hydroxide (NaOH) solution. The 20 mM NaOH solution was also used to prepare cathodes with NaOH only modification. The as-prepared solutions were sonicated for 1 hour and 285 μL of the mixture was deposited onto the buckeye paper layer (2 cm2) of the pressed air breathing cathode. The electrodes were left to dry for 4 hours at room temperature prior to being stored overnight at 4°C.

Figure 1: (a) Schematics of hematin-BOx air breathing cathode and (b) image of the biofuel cell.
3.2. Hemin and Hematin Modified Electrodes with BOx

Further investigation was carried out with BOx immobilized on hemin and hematin modified cathodes. Preparation of BOx ink was carried out as follows: 2 wt% MWNT ink solution was prepared by dissolving 4 : 1 ratio of water to ethanol (by weight) and added to 100 mg MWNTs (10–20 nm). The prepared mixture was subjected to sonication for 1 hour in ice bath vortexing every 20 min interval time. 5 μL of 300 mM Di-Carb (in DMSO) and 170 μL of stock solution of 2% MWNT ink were mixed, vortexed, and incubated for 1 hour. This was followed by addition of 5 μL of 300 mM PBSE (in DMSO) and 20 uL of water and left for incubation for 1 hour. To this, BOx (8 mg, 16 units/mL) was weighed and added to MWNT ink and incubated again for 1 hour at room temperature. Later 200 μL of the ink was then deposited on the prewetted buckeye paper of air breathing cathode modified with hemin and hematin, respectively. Control electrodes were prepared by drop casting the ink onto unmodified air breathing cathodes. Following ink deposition, a chemical deposition of tetramethyl orthosilicate (TMOS) was performed by sealing cathodes in a Petri dish containing small caps filled with water and TMOS. The Petri dish remained sealed for 5 min before discarding the TMOS. Cathodes were then stored at 4°C overnight.

3.3. Hematin/EDC-NHS Coupling Modified Electrodes with BOx

The air breathing BOx/hematin electrodes were successively modified with additional components: 1-(3-dimethylaminopropyl)-3′ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS). Hematin solution (10 mM) was prepared as described in previous section. After 1-hour sonication, 9.8 mg of EDC and NHS was added and incubated to 285 μL hematin solution. BOx ink described earlier was immobilized onto the modified hematin/EDC-NHS air breathing cathodes were left for 4 hours at room temperature prior to being stored overnight at 4°C. Figure 2 shows the schematic representation of stepwise procedure of hematin modification and BOx deposition on GDL cathode. Oxygen enters the cathode through the teflonized carbon GDL and is reduced to H2O at the hematin-BOx catalyst layer.

Figure 2: Schematic illustration of stepwise procedure of hematin modified BOx on gas diffusion layer cathode.
3.4. Electrochemical Testing

All electrochemical experiments were performed with a VMP3 potentiostat (Biologic). A conventional three-electrode system was used in the measurements with Ag/AgCl as a reference electrode, a Pt wire as the auxiliary electrode, and a bare or modified electrode as working electrode. Cyclic voltammetry was carried out and potentiostatic polarization curves were obtained to characterize half-cell testing. All tests were conducted in 245 mM, pH 7.0 PBS buffer at room temperature.

3.5. Complete Fuel Cell Testing

Glucose dehydrogenase (GDH, Toyobo) based anodes were prepared for complete fuel cell testing. Polymethylene green (PMG) was electrodeposited onto (electrode area 7.3 cm2) carbon felt electrodes following a modified version of a previously described procedure [30]. A single wall nanotube (SWNT-PEI) ink solution containing GDH was drop-casted on top of the PMG treated carbon felt electrodes. The anodes were stored overnight at 4°C prior to testing. The GDH anodes were paired with 9 cm2 (7.3 cm2 working area) hematin modified BOx or unmodified BOx cathodes for complete fuel cell testing. The fuel cells were tested with 100 mM glucose/10 mM nicotinamide adenine dinucleotide (NAD) in 245 mM PBS buffer pH 7.0. A Constant Load Discharge (CLD) technique was employed to generate power and current density curves. Later, discharge curves were generated by applying a constant load of 3.0 mA to the fuel cell. The cells were filled with fresh fuel before each subsequent discharge. Preparation of BOx ink for complete fuel cell testing was described in Supplementary File.

4. Results and Discussion

4.1. Electrochemical Testing and Characterization of Hemin and Hematin Electrodes

To better realize the function towards the enhancement of BOx cathode, hematin air breathing cathodes were prepared without BOx to determine if the electrodes were independently capable of catalyzing the ORR. This test was compared simultaneously with hemin air breathing cathode without BOx. The step potential studies were conducted to evaluate the electrodes under quiescent with no oxygen saturation conditions. At 50 mV, the current density for the hemin cathode was 0.4 mA/cm2 (Figure 3) while current density of 0.05 mA/cm2 was observed for hematin. The results showed that hemin demonstrated higher current density compared to hematin suggesting that hemin can serve as a catalyst for ORR. Several studies have identified hemin as a DET-type electrocatalyst for ORR. Ma et al. have shown that hemin modified PAMAM/MWCNT nanocomposite films on glassy carbon electrodes can act as both an electron conductor and catalytic mediator for L-tyrosine [38]. Others have identified hemin as an electrocatalyst for oxygen reduction and superoxide detection biosensors. Therefore, the increase in current density was predictable; however very little increase in current density was observed for the hematin electrodes. This suggests that hematin without the BOx does not act as an electrocatalyst by itself and cannot assist as a catalytic mediator for ORR reduction. Therefore, we may assume that hematin adsorbed onto BOx cathodes may enhance DET catalysis by acting as a natural substrate or precursor for the BOx enzyme.

Figure 3: Representative step potential curves for hemin (black square) and hematin (red circle) air breathing cathodes tested with 245 mM PBS, pH 7.0.

A cyclic voltammetry (CV) study was conducted to compare the electrochemical behavior of hematin modified air breathing cathodes with bare and EDC/NHS-hematin modified cathodes. Figure 1 (Supplementary) shows that the hematin modified air breathing cathodes had a reduction peak at around 300 mV when compared to a bare air breathing cathode. The peak current density was 3 mA/cm2 while no clear oxidation peaks have been observed. Since no distinct reversible redox reaction was observed for the hematin modified air breathing cathodes, hematin most likely acts as a natural substrate and a precursor for oxygen reduction reaction (ORR) at the BOx cathode. In literature, hematin has been identified as a potential alternative to horseradish peroxidase (HRP) for H2O2 detection [39] and catalysis of phenol compounds [40]. With further addition of crosslinking couple EDC/NHS, the reduction peak for the hematin modified electrodes has become less distinct. A high scan rate of 250 mV/s was employed for the CV study to test the stability of the EDC/NHS-hematin air breathing cathodes. Figure 2 (Supplementary) compares cycle 2 to cycle 20 for the air breathing cathodes modified with EDC/NHS-hematin. There was little variation observed between the magnitude of cycles 2 and 20. Shrinking CV curves would suggest that the EDC/NHS-hematin was being stripped from the electrode surface. The result of this study suggests that the air breathing cathodes modified with hematin/EDC/NHS were stable. The stability of the EDC/NHS-hematin electrodes was most likely due to the covalent attachment of the hematin to BOx via the EDC/NHS crosslinker. Hematin contains two carboxylic acid moieties, which allows the hematin to react with EDC and NHS further stabilizes the EDC-hematin intermediate and allows the intermediate product to form a covalent attachment to the BOx. EDC and NHS promote the reaction of carboxylic groups of hematin with amino groups of the BOx enzyme.

4.2. Electrochemical Testing and Characterization of Hemin/BOx and Hematin/BOx Electrodes

We further examined the effect of hematin, hemin, and nonmodified BOx catalyzed oxygen reduction. A comparison of the current densities from potentiostatic polarization curves is shown in Figure 4. The current density from the electrode containing BOx and hemin was 0.007 mA/cm2. The current density for BOx only was 0.32 mA/cm2, and the current density in the presence of hematin modified was 0.7 mA/cm2. For all electrodes the current densities generated at 300 mV versus sat Ag/AgCl concluded that hematin led to a significant increase in BOx performance. The polarization experiments with hematin demonstrated more than 2 times increase in the maximum current densities compared to only BOx cathodes with no enhancements (Figure 4). Hematin is a ferriprotoporphyrin-IX with a hydroxide ion bound to the ferric ion formed when hemin (ferriprotoporphyrin-IX with a chloride ion bound to ferric ion) is treated with strong NaOH solution. As mentioned previously, hemin has been studied as direct electron transfer moiety for oxygen reduction. Therefore, the function of hematin as an enhancement agent was compared with hemin and ferricyanide as electron transfer mediators (data not shown). Many such hemin related compounds have been identified in literature. Catechol, hydroquinone, pyrogallol, and bilirubin which are catalytically oxidized by BOx were employed in modification of biocathodes [28]. Recent studies have focused on incorporating specific substrates of BOx such as bilirubin, quinones, syringaldazine, and protoporphyrin derivatives such as protoporphyrin IX iron (III) (PPFeIII), protoporphyrin IX dimethyl ester (PPDE), and octaethylporphyrin (OEP) towards appropriate orientation that can be convenient for DET-type electrocatalysis [36]. However no study has been previously done on hematin as substrate for BOx. Therefore, hematin was further tested as a precursor and premodifier in the enhancement of BOx enzyme.

Figure 4: Representative step potential curves for BOx (black square), hemin-BOx (red circle), and hematin-BOx (blue triangle) air breathing cathodes tested in 245 mM PBS, pH 7.0.

To demonstrate that improved performance was due to the addition of hematin, BOx electrodes were tested with only NaOH modification, NaOH-hematin, and NaOH-EDC/NHS-hematin. Based on the results (Figure 5), treating the BOx electrode with NaOH led to enhanced air breathing cathode performance. However, performance was higher when the electrodes were modified with hemin in NaOH. Increased current densities achieved with NaOH modification were most likely due to the electrode surface becoming more hydrophilic, which would create a more favorable environment for the ORR taking place at the electrode surface. The highest current densities were achieved when the crosslinking couple EDC/NHS were added to the hematin solution. Current densities greater than 0.8 mA/cm2 were achieved by modifying the electrode surface with the EDC/NHS-hematin solution. All BOx cathode types were tested in triplicate. The EDC/NHS BOx air breathing cathodes performed better than the BOx only (no enhancements) cathodes at 300 mV but performed significantly less than the NaOH, NaOH-hematin, and NaOH (EDC/NHS)-hematin-BOx cathodes. The NaOH (EDC/NHS)-hematin-BOx cathodes remained the highest performing cathodes.

Figure 5: Representative step potential curves for BOx air breathing cathodes modified with NaOH only (black square), NaOH-hematin (red circle), and NaOH (EDC/NHS)-hematin (blue triangle) or no enhancement (green inverted triangle) tested in 245 mM PBS, pH 7.0.
4.3. BOx Loading Optimization

High enzyme loadings are required for the optimal fuel cell performance. However, when the enzyme loadings are too high, substrate diffusion and product inhibition can limit the rate of overall processes. To minimize the mass diffusion limitations, the BOx loading on hematin modified air breathing cathodes was optimized. The enzyme loading ranging from 1 mg/cm2 to 14 mg/cm2 was tested (Figure 6). The results show increase in current densities with increase in BOx loading on the cathode. Since the initial rate of reaction of the enzyme corresponds to the activity and amount of enzyme loaded, the current increased until optimal loading of 10 mg/cm2 has reached and decreased when the electrode surface was loaded with 14 mg/cm2 enzyme. This could be due to diffusion limitations where part of the immobilized enzyme may be not being available for catalysis. Figure 7 shows the linear dependence of current density at 300 mV with the BOx loading ranging from 1 mg/cm2 to 10 mg/cm2. It is to be noted that non-hematin-BOx cathodes performed optimally at 14 mg/cm2 BOx loading and by employing hematin, BOx loading was reduced by 29%. At 300 mV, the optimized hematin-BOx cathode performed twice better than the non-hematin-BOx cathode (Figure 8).

Figure 6: Representative step potential curves for BOx air breathing cathodes prepared with different amounts of BOx ranging from 1 mg/cm2 to 14 mg/cm2 tested in 245 mM PBS, pH 7.0.
Figure 7: Linear regression plot for current density as a function of BOx loading.
Figure 8: Representative step potential curves for optimized BOx air breathing cathodes prepared without hematin (black square) or with hematin (red circle) tested in 245 mM PBS, pH 7.0.
4.4. Complete Fuel Cell Testing

A DET-type biofuel cell without separators was constructed. The biocathode was an air breathing hematin/BOx and the bioanode was GDH/PMG carbon felt electrodes. Complete fuel cell testing was conducted with 100 mM glucose/10 mM NAD fuel in 245 mM PBS buffer pH 7.0 under quiescent conditions at room temperature. The current densities dependence of the cell power density is shown in Figure 9(a) while Figure 9(b) shows the maximum current densities for the hematin modified BOx cathodes. The open circuit potential of 0.7 V was obtained. The maximum current density was 6.6 mA/cm2 and maximum power density was 1.1 mW/cm2 at 300 mV of the fuel cell. On the other hand, with non-hematin modified cathode the maximum current density of 2.5 mA/cm2 and maximum power density of 0.2 mW/cm2 were obtained. From the results the determining factor for the high current density is controlled by the BOx cathode. The performance of the hematin-BOx cathodes was 2.4 times higher than the performance for the BOx cathodes without hematin modification. Constant current load discharge studies of the hematin/BOx cathode and GDH anodes based biofuel cells were further conducted with 50 mM glucose/10 mM NAD fuel in 245 mM PBS buffer pH 7.0. A constant current load of 3.0 mA was applied to each fuel cell until the potential reached 350 mV. With injection of fresh fuel, the fuel cell was tested under repeated discharge cycles. A total of 4 discharges were performed consecutively with 10-minute recovery periods between discharges as shown in Figure 3 (Supplementary). The average runtime varied between 0.76 h and 1.0 h with average capacity ranging from 126 mAh/g to 167 mAh/g. The capacity of the BOx/GDH fuel cells decreased by less than 25% after being discharged to 350 mV multiple times. The results suggest that the fuel cells can withstand multiple discharges (with fresh fuel) without experiencing a significant loss in capacity. These results also indicate that there was minimal leaching out of adsorbed functionalities of anode and cathode biomaterials.

Figure 9: Representative power density (a) and current density (b) curves for optimized BOx air breathing cathodes prepared without hematin (black square) or with hematin (red circle) paired with GDH anodes tested in 100 mM glucose.

5. Conclusion

In this study, direct interaction of BOx on hematin modified MWCNT is capable of increasing the current density driven by a combination of synchronous effect of natural substrate and favorable orientation for BOx. With hematin based electrode surface modification the optimized loading of BOx was drastically decreased by 28.6%, and an average peak power density above 1 mW/cm2 was achieved. Using hematin, it was investigated that the assembly of hematin with EDC/NHS resulted in significant increase of current density compared to the non-hematin modified electrode. The hematin modified electrode showed remarkable stability when subjected to more than 15 cycles of cyclic voltammetry. The current density as high as 6.6 mA/cm2 at 300 mV and power density of 1.1 mW/cm2 were obtained with GDH/O2 biofuel cell.


The content of this manuscript does not necessarily reflect the position or the policy of the Government, and no official endorsement should be inferred. Abstract on this work was submitted at 233rd ECS Meeting.

Conflicts of Interest

The authors declare no conflicts of interest.


This research was supported by the U.S. Army Research Office (STTR Contract W911NF-13-C-0015).

Supplementary Materials

Supplementary materials contain the procedure for preparation of bilirubin oxidase (BOx) ink for complete fuel cell testing. The cyclic voltammetry studies for testing the bare, hematin modified, and EDC/NHS-hematin modified electrode are shown in Supplemental Figure 1 and showed the stability of hematin modified electrode (Supplemental Figure 2). Recycling and discharge studies under constant current load of GDH/BOx fuel cell were shown in Supplemental Figure 3. (Supplementary Materials)


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