The Role of Immune Correlates and Surrogate Markers in the Development of Vaccines and Immunotherapies for Plague
The cytoxicity of Y. pestis V antigen expressed from Y. pseudotuberculosis for J774 cells was inhibited by pretreatment with anti-V Mab 7.3. Cells were stained with ethidium bromide/acridine orange to identify live cells (green) and dead cells (red). (a) Unifected J774 cells, (b) J774 cells infected with Y. pseudotuberculosis expressing V antigen were killed, shown by the preponderance of dead cells. (c) J774 cells pretreated with Mab 7.3 prior to exposure to Y. pseudotuberculosis expressing V antigen were protected, with no significant difference in appearance, compared with uninfected cells.
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