Methods to Evaluate the Effect of Ethanol on the Folate Analogue: Fluorescein Methotrexate Uptake in Human Proximal Tubular Cells
Figure 1
Confocal images of FL-MTX uptake by HPT cells in 24 well plates. Cellular fluorescence was more when the cells treated with 4 M FL-MTX and it was gradually decreasing when the cells were treated with 2 M, 1 M, and 0.5 M FL-MTX. Thus, FL-MTX uptake was directly proportional to its concentrations.
