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Advances in Pharmacological Sciences
Volume 2013 (2013), Article ID 101759, 7 pages
Research Article

In Vivo Potential Anti-Inflammatory Activity of Melissa officinalis L. Essential Oil

1Laboratory of Pharmacology and Toxicology, Department of Drugs Sciences, Faculty of Medicine and Pharmacy, Mohammed V Souissi University, Rabat Instituts, BP 6203, Agdal, Rabat, Morocco
2Laboratory of Biochemistry and Immunology, Department of Biology, Faculty of Science, Mohammed V Agdal University, Rabat Instituts, BP 6203, Agdal, Rabat, Morocco
3Departement of Pharmacology, University of Thamar, BP 87246, Thamar, Yemen

Received 3 October 2013; Accepted 5 November 2013

Academic Editor: Eduardo Munoz

Copyright © 2013 Amina Bounihi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Melissa officinalis L. (Lamiaceae) had been reported in traditional Moroccan medicine to exhibit calming, antispasmodic, and strengthening heart effects. Therefore, this study is aimed at determining the anti-inflammatory activities of M. officinalis L. leaves. The effect of the essential oil of the leaves of this plant was investigated for anti-inflammatory properties by using carrageenan and experimental trauma-induced hind paw edema in rats. The essential oil extracted from leaves by hydrodistillation was characterized by means of gas chromatography-mass spectrometry (GC-MS). M. officinalis contained Nerol (30.44%), Citral (27.03%), Isopulegol (22.02%), Caryophyllene (2.29%), Caryophyllene oxide (1.24%), and Citronella (1.06%). Anti-inflammatory properties of oral administration of essential oil at the doses of 200, 400 mg/kg p.o., respectively, showed significant reduction and inhibition of edema with 61.76% and 70.58%, respectively, ( ) induced by carrageenan at 6 h when compared with control and standard drug (Indomethacin). On experimental trauma, M. officinalis L. essential oil showed pronounced reduction and inhibition of edema induced by carrageenan at 6 h at 200 and 400 mg/kg with 91.66% and 94.44%, respectively ( ). We can conclude that the essential oil of M. officinalis L. possesses potential anti-inflammatory activities, supporting the traditional application of this plant in treating various diseases associated with inflammation and pain.