Silicone-Acyclovir Controlled Release Devices Suppress Primary Herpes Simplex Virus-2 and Varicella Zoster Virus Infections In Vitro
Figure 2
Comparison of in vitro antiviral activity of implants made from two different silicone polymers impregnated with ACV. Vero cells were grown to near-confluence in 24-well culture plates. One implant was placed in each well. Two days later, either medium alone (“Mock”) or HSV-2 (G) (95 pfu) was added to the indicated wells. (a) Digital photographs were taken 3 days after infection. Wells in the first column (“None”) received neither implant nor ACV. The remaining wells received MED-4050 or MED-4750 impregnated with ACV, as indicated. Each implant was prepared by mixing polymer component A, polymer component B, and ACV in a 1 : 1 : 1 ratio as described in Section 2. Supernatants (200 μL) were removed from the culture 3 days after infection. (b) A portion of each captured supernatant (100 μL) was used to determine HSV-2 titer. (c) ACV was quantified from the other portion (100 μL) of the recovered supernatant.
