Silicone-Acyclovir Controlled Release Devices Suppress Primary Herpes Simplex Virus-2 and Varicella Zoster Virus Infections In Vitro

Figure 4

Comparison of in vitro release of acyclovir (ACV) from implants made from MED-4050 and MED-4750 polymers. Silicone rods generated from either MED-4050 or MED-4750 polymers were impregnated with ACV in a 1 : 1 : 1 ratio of component A, component B, and ACV, and the release of acyclovir was determined every 24 hours for 60 days. Inset shows a magnified view of days 11 to 60, after initial burst release of ACV. The rate of release of ACV from the implants was determined as described in Section 2. Briefly, implants were suspended in 1 mL of phosphate-buffered saline and removed to fresh PBS every 24 hours. Following incubation, each sample was analyzed by HPLC. ACV was quantitated based on comparison with a standard curve. Each point represents the average of five independent samples; error bars denote standard deviation of the averages.