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Advances in Pharmacological Sciences
Volume 2014, Article ID 416761, 7 pages
http://dx.doi.org/10.1155/2014/416761
Research Article

Development of an Antioxidant Phytoextract of Lantana grisebachii with Lymphoprotective Activity against In Vitro Arsenic Toxicity

1Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, INICSA-CONICET/UNC, Enrique Barros S/N, 5014 Córdoba, Argentina
2Consejo Interuniversitario Nacional, Pacheco de Melo 2084, 1126 Ciudad Autónoma de Buenos Aires, Argentina
3Facultad de Agronomía y Veterinaria, Universidad Nacional de Río Cuarto, IMBIV-CONICET/UNC, Ruta 36 Km 601, 5804 Río Cuarto, Argentina
4Facultad de Ciencias Agrarias, Universidad Nacional del Comahue, PROBIEN-CONICET/UNCO, CP 8300, Neuquén, 1400 Buenos Aires, Argentina

Received 25 January 2014; Revised 5 May 2014; Accepted 16 May 2014; Published 5 June 2014

Academic Editor: Robert Gogal

Copyright © 2014 Elio A. Soria et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Phytochemicals have been presumed to possess prophylactic and curative properties in several pathologies, such as arsenic- (As-) induced immunosuppression. Our aim was to discover a lymphoprotective extract from Lantana grisebachii Stuck. (Verbenaceae) (LG). We assessed its bioactivity and chemical composition using cell-based assays. Fractions produced from a hexane extract acutely induced nitrite formation in T-activated cell cultures . Water extraction released a fraction lacking nitrite inducing activity in both lymphocyte types. Aqueous LG was found to be safe in proliferated and proliferating cells. The infusion-derived extract presented better antioxidant capacity in proportion to phenolic amount in lymphocytes (infusive LG-1i at 100 μg/mL), which protected them against in vitro As-induced lymphotoxicity . This infusive LG phytoextract contained  mg/g of phenolics, with 58.46% being flavonoids. Among the phenolics, the only predominant compound was 0.723 mg of chlorogenic acid per gram of dry plant, in addition to 10 unknown minor compounds. A fatty acid profile was assessed. It contained one-third of saturated fatty acids, one-third of ω9, followed by ω6 (~24%) and ω3 (~4%), and scarce ω7. Summing up, L. grisebachii was a source of bioactive and lymphoprotective compounds, which could counteract As-toxicity. This supports its phytomedical use and research in order to reduce As-related dysfunctions.