Antileishmanial Effect of 5,3′-Hydroxy-7,4′-dimethoxyflavanone of Picramnia gracilis Tul. (Picramniaceae) Fruit: In Vitro and In Vivo Studies

Table 1

In vitro cytotoxicity and antileishmanial activity of Picramnia gracilis Tul.

Product	Biological activity (μg/mL)		IS
	LC50	EC50
E-EtOH-le	>200.0	>100.0	>2
E-He-le	22.4 ± 5.4	>22.4	<1
E-DiCl-Me-le	29.1 ± 9.0	>29.1	<1
E-EtAc-le	>200.0	>100.0	>2
E-EtOH-fr	>200.0	35.7 ± 1.3	>2
5,3′-Hydroxy-7,4′-dimethoxyflavanone	>200.0	17.0 ± 2.8 (53.7 μM)	>11.8
Amphotericin B	37.5 ± 7.6	0.06 ± 0.004	625.0
Meglumine antimoniate	>1000.0	6.8 + 0.5	>147.1

Data represent the mean value ± SD of cytotoxicity in terms of 50% lethal concentration (LC₅₀) and leishmanicidal activity in terms of 50% effective concentration (EC₅₀). E-EtOH-fr: ethanolic extract from fruit; E-EtOH-le: ethanolic extract from leaves; E-DiClMe-le: dichloromethane extract from leaves; E-He-le: hexane extract from leaves; and E-EtAc-le: ethyl acetate extract from leaves. IS (index of selectivity) = LC₅₀/EC₅₀.