(i) Chromatography (silica gel column or AB-8 resin column) (ii) HPLC (iii) HPTLC
(i) 49 mg of orientin from the AOB concentrated solution (ii) 6.44%, 7.82%, and 4.92% (w/w) of orientin were produced from P. pubescens, P. glauca, and P. yixingensis, respectively, by HPTLC
(i) HPLC (ii) Column chromatography (iii) HSCCC (iv) UPLC-ESI-MS/MS
(i) 5 mg of orientin from 15 kg of
T. ledebouri
dried flowers (ii) 95.8 mg of orientin from 500 mg of the crude extract of T. ledebouri (iii) Able to measure minimum 515 g of orientin per gram of plant material by UPLC-ESI-MS/MS method
Water, dichloromethane, ethyl acetate, n-butanol, or residual aqueous
(i) TLC (ii) HPLC
(i) The retention factor of orientin is 0.52 for TLC analysis (ii) Solve the problem of overlapping of the isomers (orientin and homorientin) chromatographic bands in HPLC