Effects of Curcumin on Parameters of Myocardial Oxidative Stress and of Mitochondrial Glutathione Turnover in Reoxygenation after 60 Minutes of Hypoxia in Isolated Perfused Working Guinea Pig Hearts

Table 3

Mitochondrial parameters.

	Normoxia	Hypoxia	Reoxygenation
MDA: nmol × mg^−1 mitochondrial protein (; mean ± SD; N versus R and R versus R0.25  )
	17.85 ± 2.79 (100%)	21.24 ± 3.53 (119.0%)	29.52 ± 9.47 (165.4%; N versus R; )
Curc. 0.25 µM		17.68 ± 4.40 (99.1%)	16.47 ± 1.99 (92.3%; R versus R0.25; )
Curc. 0.5 µM		19.93 ± 3.62 (111.7%)	24.45 ± 3.24 (137.0%)
GSH: nmol × mg^−1 protein ( in each group; mean ± SD)
	40.6 ± 10.9 (100%)	22.9 ± 10.7 (56.4%)	14.3 ± 4.3 (35.2%)
		N versus H ()	N versus R ()
Curc. 0.25 µM		⁢19.5 ± 7.1 (48.0%)	27.0 ± 11.2 (66.5%)
			R versus R0.25 ()
Curc. 0.5 µM		⁢23.2 ± 7.0 (57.1%)	28.3 ± 8.0 (69.7%)
			R versus R0.5 ()
GPx: nmol × min^−1  ×  mg^−1 protein ( in each group; mean ± SD)
	324.1 ± 115.0 (100%)	392.2 ± 150.1 (121.1%)	826.1 ± 268.0 (255.2%)
		H versus R ()	N versus R ()
Curc. 0.25 µM		⁢513.3 ± 59.4 (158.6%)	498.6 ± 44.5 (153.8%)
			R versus R0.25 ()
Curc. 0.5 µM		664.4 ± 206.3 (205.3%)	665.0 ± 219.1 (205.7%)
GR: nmol × min^−1  ×  mg^−1 protein ( in each group; mean ± SD)
	77.2 ± 15.7 (100%)	45.3 ± 17.1 (58.8%)	22.2 ± 9.6 (29.3%; N versus R )
Curc. 0.25 µM		36.9 ± 16.9 (48.8%)	35.4 ± 16.0 (46.8%)
Curc. 0.5 µM		47.3 ± 18.6 (62.5%)	46.9 ± 18.5 (62.0%; R versus R0.5  )

MDA, malondialdehyde; GSH, reduced glutathione; GPx, glutathione peroxidase; GR, glutathione reductase; N, normoxia; H, hypoxia; R, reoxygenation; , reoxygenation with 0.25 µM curcumin (curc.); , reoxygenation with 0.5 µM curc.