Chemical Composition and Cytotoxic and Antibacterial Activities of the Essential Oil of Aloysia citriodora Palau Grown in Morocco

Table 1

Chemical composition after GC-MS analysis of the essential oils of A. citriodora.

Peak number	Component	RI	% AG	% BM	% BE	% DE	% MA	Identification
1	α-Pinene	909	0.23	0.39	0.17	0.21	0.20	RI, MS
2	α-Thujene	927	0.09	0.14	0.08	0.09	0.08	RI, MS
3	β-Pinene	964	0.14	0.14	0.09	0.12	0.09	RI, MS
4	Sabinene	972	0.04	0.05	0.03	0.04	0.04	RI, MS
5	6-Methyl-5-hepten-2-one	987	0.03	0.02	0.02	0.02	0.02	RI, MS
6	β-Myrcene	988	0.03	0.02	0.02	0.02	0.04	RI, MS
7	α-Terpinene	1017	0.12	0.10	0.08	0.08	0.09	RI, MS
8	Paracymene	1024	0.02	—	—	—	—	RI, MS
9	Limonene	1027	0.71	0.63	0.36	0.5	0.39	RI, MS
10	1,8-Cineole	1031	1.97	1.44	1.66	1.41	1.97	RI, MS
11	cis-β-Ocimene	1037	0.02	0.02	—	0.02	0.01	RI, MS
12	trans-β-Ocimene	1045	0.04	0.03	0.05	0.03	0.05	RI, MS
13	-Terpinene	1054	0.33	0.13	0.15	0.18	0.14	RI, MS
14	trans-Sabinene hydrate	1096	0.29	0.14	0.17	0.17	0.13	RI, MS
15	6-Camphenol	1110	0.08	0.04	0.05	0.06	0.05	RI, MS
16	cis-Limonene oxide	1117	0.16	0.10	0.14	0.13	0.22	RI, MS
17	Campholene aldehyde	1125	0.58	0.26	0.4	0.4	0.40	RI, MS
18	trans-p-Mentha-2,8-dienol	1127	0.18	0.17	0.16	0.10	0.12	RI, MS
19	cis-p-Mentha-2,8-dien-1-ol	1131	0.33	0.18	0.20	0.21	0.15	RI, MS
20	trans-Verbenol	1144	0.64	0.34	0.47	0.38	0.45	RI, MS
21	cis-Verbenol	1153	7.78	4.87	5.22	5.82	4.41	RI, MS
22	1,3,4-Trimethyl-3-cyclohexene-1-carboxaldehyde	1171	0.70	0.63	0.59	0.53	0.62	RI, MS
23	cis-p-Mentha-1(7),8-dien-2-ol	1185	0.13	0.09	0.14	0.07	0.07	RI, MS
24	Verbenyl ethyl ether	1186	0.31	0.30	0.24	0.35	0.31	RI, MS
25	2-Carene	1189	1.71	0,86	1.11	1.03	0.84	RI, MS
26	Myrtenol	1193	0.58	0.33	0.36	0.37	0.32	RI, MS
27	trans-2-Caren-4-ol	1222	0.53	0.29	0.43	0.33	0.42	RI, MS
28	D-Carvone	1223	0.27	0.22	0.26	0.19	0.34	RI, MS
29	cis-Carveol	1226	0.06	0.03	0.04	0.05	0.03	RI, MS
30	Nerol	1228	1.85	2.16	1.56	2.05	1.60	RI, MS
31	Neral	1242	10.02	7.23	8.57	8.18	6.37	RI, MS
32	Piperitone	1250	0.15	0.11	0.14	0.12	0.07	RI, MS
33	2,6,6-Trimethyl-1-cyclohexene-1-acetaldehyde	1254	0.27	0.12	0.19	0.18	0.15	RI, MS
34	Perillic aldehyde	1257	0.03	0.03	0.02	0.03	0.04	RI, MS
35	para-Cymen-7-ol	1289	0.05	0.09	0.13	0.05	0.10	RI, MS
36	8,11,14-Eicosatrienoic acid. (Z.Z.Z)-	1294	0.04	0.05	0.03	0.03	0.03	RI, MS
37	1-(1,3-Dimethyl-buta-1.3-dienyl)-3,7,7-trimethyl-2-oxa- bicyclo[3.2.0]hept-3-ene	—	0.03	0.03	0.03	0.03	0.06	RI, MS
38	trans-Carvyl acetate	1342	0.07	0.07	0.07	0.08	0.04	RI, MS
39	Eugenol	1356	0.19	0.22	0.20	0.21	0.31	RI, MS
40	Geranyl acetate	1365	0.66	1.22	0.91	1.29	1.06	RI, MS
41	α-Copaene	1375	1.25	1.73	1.27	1.45	2.16	RI, MS
42	Isoledene	1377	3.48	4.46	3.89	4.19	4.04	RI, MS
43	7-Tetracyclo [6.2.1.0 (3.8)0(3.9)] Undecanol, 4,4,11,11-tetramethyl-	—	0.10	0.17	0.12	0.14	0.13	RI, MS
44	Di-epi-α-cedrene	1412	0.67	0.96	0.67	0.82	0.68	RI, MS
45	β-Caryophyllene	1420	3.11	3.26	2.77	4.18	1.85	RI, MS
46	ç-Elemene	1433	2.30	3.04	2.77	2.99	2.93	RI, MS
47	trans-α-Bergamotene	1434	0.09	0.09	0.08	0.09	0.04	RI, MS
48	Aromadendrene	1440	0.06	0.05	0.06	0.1	0.05	RI, MS
49	α-Humulen	1453	0.6	0.49	0.59	0.61	0.20	RI, MS
50	Alloaromadendrene	1460	1.66	1.36	1.02	1.30	1.00	RI, MS
51	Germacrene-D	1480	0.28	0.39	0.29	0.36	0.31	RI, MS
52	Ar-curcumene	1483	11.47	13.38	11.28	14.15	11.30	RI, MS
53	β-Guaiene	1490	0.01	0.01	0.02	0.02	0.01	RI, MS
54	Tricyclo[5.2.2.0(1.6)]undecan-3-ol, 2-methylene-6,8,8-trimethyl-	1498	0.49	0.68	0.56	0.56	0.65	RI, MS
55	α-Muurolene	1499	—	0.04	—	0.04	—	RI, MS
56	ç-Himachalene	1505	0.34	0.60	0.47	0.46	0.64	RI, MS
57	Calamenene	1514	0.09	0.16	0.11	0.12	0.13	RI, MS
58	9-Isopropyl-1-methyl-2-methylene-5-oxatricyclo[5.4.0.0(3,8)]undecane	1522	0.05	0.09	0.07	0.08	0.09	RI, MS
59	-Cadinene	1523	0.17	0.26	0.19	0.23	0.22	RI, MS
60	ç-Cadinene	1524	3.89	5.04	4.57	4.99	4.86	RI, MS
61	β-Spathulenol	1576	13.27	10.19	15.61	9.42	13.42	RI, MS
62	trans-Caryophyllene oxide	1580	13.52	13.25	14.14	13.28	14.22	RI, MS
63	Ledene oxide-(I)	1631	1.75	2.96	2.41	2.25	3.01	RI, MS
64	8-Cedren-13-ol	1657	—	0.02	0.03	0.01	0.05	RI, MS
65	Alloaromadendrene oxide-(1)	1672	3.06	4.26	4.37	3.79	4.35	RI, MS
66	Alloaromadendrene oxide-(2)	1678	0.28	0.50	0.4	0.4	0.79	RI, MS
67	Eudesma-4,11-dien-2-ol	1690	0.31	0.46	0.46	0.45	0.62	RI, MS
68	trans-Nuciferol	1727	2.31	3.10	2.60	3.10	3.86	RI, MS
69	cis-Nuciferol	1734	2.17	3.30	2.32	2.80	3.17	RI, MS
70	Murolan-3,9(11)-diene-10-peroxy	—	0.36	0.53	0.5	0.48	0.73	RI, MS
71	Ledene oxide-(II)	2062	0.35	0.45	0.47	0.45	0.57	RI, MS
72	Tricyclo[5.2.2.0(1.6)]undecan-3-ol, 2-methylene-6,8,8-trimethyl-	—	0.97	1.25	1.31	1.33	1.35	RI, MS

Compounds are listed in order of their elution from a DB-5 column. Linear retention index taken from NIST 08, National Institute of Standards and Technology, Mass Spectral Library (NIST/EPA/NIH). Essential oil of Agadir. Essential oil of Beni Mellal. Essential oil of Berkane. Essential oil of Demnate. Essential oil of Marrakech.