Advances in Pharmacological and Pharmaceutical Sciences

Research Article

HPTLC Analysis of Solanum xanthocarpum Schrad. and Wendl., a Siddha Medicinal Herb

Table 1

Data showing bioactive markers, composition of solvent system, derivatizing reagent used and wavelength of all the marker compounds used in the present study for HPTLC analysis.
S. no.Bioactive compoundSolvent systemComposition (v/v/v/v)Derivatizing reagentWavelength (nm)
Triterpenoids     
1.LupeolToluene:methanol:formic acid9 : 4: 0.2p-Anisaldehyde sulphuric acid530
2.Oleanolic acidToluene: ethylacetate:formic acid7 : 3 : 0.3p-Anisaldehyde sulphuric acid540
3.Ursolic acidToluene: ethylacetate:formic acid8 : 2 : 0.1p-Anisaldehyde sulphuric acid510
Phytosterols     
4.β-SitosterolToluene: ethylacetate9 : 4p-Anisaldehyde sulphuric acid530
5.CampesterolToluene:methanol:formic acid9 : 4 : 0.2p-Anisaldehyde sulphuric acid530
6.ErgosterolToluene:methanol:formic acid9 : 4 : 0.2p-Anisaldehyde sulphuric acid530
7.Withanolide BToluene:methanol:formic acid9 : 4 : 0.2p-Anisaldehyde sulphuric acid530