Antipathogenic Potential of a Polyherbal Wound-Care Formulation (Herboheal) against Certain Wound-Infective Gram-Negative Bacteria

Figure 4

(a) HF enhances catalase activity, inhibits haemolytic potential, and increases susceptibility of P. aeruginosa to lysis by human serum: the catalase assay was done by monitoring disappearance of H₂O₂ at 240 nm. Tetracycline (0.5 μg/mL) inhibited catalase activity of this bacterium by 21.51% ± 0.02. Hemoglobin concentration was measured at OD₅₄₀. “Control” in the serum-dependent lysis assay was HF-unexposed cells of P. aeruginosa incubated with human serum. (b) HF treatment reduces the virulence of P. aeruginosa towards C. elegans: catechin (50 μg/mL) and gentamicin (0.1 μg/mL) employed as positive controls conferred 100% and 80% protection, respectively. Pretreatment of bacteria with HF at 0.025% v/v, 0.1% v/v, and 1% v/v conferred 70%, 55%, and 45% survival benefit, respectively. Survival benefit refers to the difference between the number of worms surviving in experimental and control wells. HF at tested concentrations showed no toxicity towards C. elegans. ; ; ; HF: Herboheal formulation.

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