Research Article

Antiaging and Antioxidant Bioactivities of Asteraceae Plant Fractions on the Cellular Functions of the Yeast Schizosaccharomyces pombe

Table 2

Primer pairs used in the analysis of gene expression by using qRT-PCR.

Targeted genesPrimer sequence

Reference gene (act1+)
Forward (F)5′ CGGTCGTGACTTGACTGACT 3′
Reverse (R)5′ ATTTCACGTTCGGCGGTAGT 3′

Transcriptional factor Pap1 (pap1+)
Forward (F)5′ TGGATGGCGATGTTAAGCCT 3′
Reverse (R)5′ GCAGCACGGTTTTGAGCTTT 3′

Superoxide dismutase 2 (sod2+)
Forward (F)5′ ATTTGGAGGGAGAGGTTGCC 3′
Reverse (R)5′ GATTGATGTGACCACCGCCA 3′

Catalase (ctt1+)
Forward (F)5′ TCGTGACGGCCCTATGAATG 3′
Reverse (R)5′ AGCAAGTGGTCGGATTGAGG 3′