Development and Characterization of PEGylated Chromatographic Monoliths as a Novel Platform for the Separation of PEGylated RNase a Isomers

Table 2

Retention time and resolution factor (Rs) of PEGylated Ribonuclease A using PEGylated monoliths.

PEG MW in PEGylated monolith (kDa)	PEGylated Ribonuclease A
	5 kDa			20 kDa			40 kDa
	Retention time (mL)		Rs	Retention time (mL)		Rs	Retention time (mL)		Rs
	Mono-PEG	Di-PEG		Mono-PEG	Di-PEG		1st peak	2nd peak
1	12.35	13.46	0.62 ± 0.03	14.95	16.7	0.94 ± 0.02	NA	NA	NA ± NA
10	12.83	14.19	0.95 ± 0.02	15.4	16.72	1.10 ± 0.04	NA	NA	NA ± NA
20	12.92	14.35	1.02 ± 0.01	15.94	17.17	1.04 ± 0.01	17.05	17.6	0.76 ± 0.01