Development and Characterization of PEGylated Chromatographic Monoliths as a Novel Platform for the Separation of PEGylated RNase a Isomers

Table 3

Retention time and resolution factor (Rs) of PEGylated Ribonuclease A using combined PEGylated monoliths in series.

PEGylated monoliths order (kDa)	PEGylated Ribonuclease A
	5 kDa			20 kDa			40 kDa
	Retention time (mL)		Rs	Retention time (mL)		Rs	Retention time (mL)		Rs
	Mono-PEG	Di-PEG		Mono-PEG	Di-PEG		1st peak	2nd peak
10-20	13.41	14.79	1.06 ± 0.01	16.2	17.31	1.07 ± 0.02	17.30	17.73	0.67 ± 0.04
20-10	13.44	14.82	1.03 ± 0.01	16.22	17.33	0.97 ± 0.03	17.31	17.74	0.57 ± 0.05
1-10-20	13.76	15.08	1.06 ± 0.01	16.52	17.55	0.98 ± 0.04	17.53	17.97	0.52 ± 0.02
20-10-1	13.71	15.01	0.99 ± 0.01	16.46	17.53	0.85 ± 0.09	17.52	17.91	0.45 ± 0.00