Freeze-Concentrated Phase and State Transition Temperatures of Mixtures of Low and High Molecular Weight Cryoprotectants

Table 2

Uncoded regression coefficients and variance analysis of the hierarchical mathematical models to evaluate the variation of response variables as a function of chemical composition of the cryoprotectant solutions (p<0.05).

Response variables
Effect
	values	p>t	values	p>t	values	p>t
	-150.42	< 0.0001	-25.84	< 0.0001	542.57	< 0.0001
	-51.85	< 0.0001	-42.15	< 0.0001	-425.77	0.2184
	109.26	< 0.0001	-154.73	< 0.0001	-364.76	< 0.0001
	-50.10	< 0.0001	-19.77	< 0.0001	490.96	< 0.0001
	127.35	< 0.0001	261.83	0.6304	233.10	0.3608
	-61.02	0.1873	111.71	0.1788	-45.29	0.8449
	-272.34	0.0190	279.03	0.9517	777.85	0.0197
	397.90	0.0008	69.14	0.0343	-314.98	0.2683
	-13.08	0.7631	-24.86	0.3756	335.40	0.2069
	-35.29	< 0.0001	-51.63	0.5986	-3827.75	0.0559
	-427.34	0.0296	-1642.90	< 0.0001	-22925.84	< 0.0001
	-580.60	0.0160	-672.41	0.0057	-
	-369.15	0.0817	345.36	0.1057	10794.51	0.0052
	-281.90	0.3225	-	-	-
	-156.36	0.0171	-	-	-
	-271.59	0.0002	-137.14	0.0201	-2310.27	0.0241
	166.11	0.1724	-	-
	130.49	0.0225	183.97	0.0037	2801.85	0.0079
	-	-	-	-	-	-
	-328.21	0.0311	234.05	0.0942	-	-
Models
p>F	< 0.0001		< 0.0001		< 0.0001
R^2	0.9971		0.9907		0.9849
Lack of fit	0.2205		0.1066		0.2302
C.V. (%)	3.38		5.90		8.57

Note: according to a Box-Cox analysis, a transformation Tm = (Tm + 20)² on Tm values was carried out to stabilize the variance.