3D In Vitro Human Organ Mimicry Devices for Drug Discovery, Development, and Assessment

Figure 13

Gut-on-a-chip microfluidic device. (a) Photograph and (b) schematic of the device. (c) Micrograph of human Caco-2 intestinal epithelial cells cultured in the device. (d) Apparent permeability (Papp) of the epithelium assessed by adding fluorescent inulin-FITC. (e) Confocal immunofluorescence micrograph of human villus intestinal epithelium formed inside the gut-on-a-chip and stained for villin (yellow). (f) SEM micrographs of the apical surface of the villus epithelium. Reproduced (or adapted) with permission [85], ©2017, Public Library of Science.

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