Recent Advances in Carbon Nanotubes for Nervous Tissue Regeneration

Figure 3

(a) Molecular representation of graphene [37]. (b) AFM topography of single-layer graphene (SGL, scale bar: 5 μm). (c) Scanning electron micrograph depicting hippocampal neuron morphology cultured onto SLG (scale bar: 10 μm). (d) Fluorescent microscopy images showing dissociated hippocampal networks labelled with class III β-tubulin (for neurons) in red and GFAP (for astrocytes) in green (scale bar: 100 μm). (e) Sketch of the local amount of K+ depletion in the space between the cell membrane and the SLG surface (membrane/surface cleft) due to graphene trapping as function of cleft thickness. The light green region shows the extrapolated K+ depletion values (red line) within the range of the estimated cleft dimensions (40–100 nm). See Reference [35] for more details.

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