Identification of Residues Important for the Activity of Haloferax volcanii AglD, a Component of the Archaeal N-Glycosylation Pathway

Figure 2

Conserved residues in archaeal AglD proteins. The sequences of Hfx. Volcanii  AglD (accession number AM698042) and AglD homologues in Archaeoglobus fulgidus (NP_069415.1; Aful), Aeropyrum pernix (NP_147774.1; Aper), Haloarcula marismortui (YP_136461.1; Hmar), Halobacterium sp. NRC-1 (NP_279416.1; HNRC), Haloquadratum walsbyi (YP_657261.1; Hwal), Methanosarcina acetivorans (NP_618739.1; Mace), Methanosarcina barkeri (YP_304067.1; Mbar), Methanospirillum hungatei (YP_503949.1; Mhun), Methanopyrus kandleri (NP_614163.1; Mkan), Metallosphaera sedula (YP_001191894; Msed), Natronomonas pharaonis (YP_326773.1; Npha), Pyrococcus abyssi (NP_127133.1; Paby), Picrophilus torridus (YP_024256.1; Ptor), Sulfolobus solfataricus (NP_342803.1; Ssol), Thermococcus kodakarensis (YP_182777.1; Tkod), and Thermoplasma volcanium (NP_111403.1; Tvol) were aligned by ClustalW2 (www.ebi.ac.uk/Tools/clustalw2/index.html), using the default settings. The region of the highest similarity is shown. Completely conserved residues are shown against a black background, while largely conserved residues (i.e., similar residues conserved in at least 11 sequences) are shown against a grey background. Amino acid numbers are shown at the start and end of each sequence. Asterisks are placed under Hfx. volcanii AglD D110, D112, R139, D173, D195, and D201 (see text for details).