Mutational and Bioinformatic Analysis of Haloarchaeal Lipobox-Containing Proteins
Figure 4
Cysteine to serine mutants of Hvo_1808, Hvo_1580, and Hvo_0494 are less stable than wild-type constructs but appear to be processed. Western blot analyses of wild-type proteins (WT), cysteine to serine replacement mutants (Hvo_1808C19S, Hvo_1580C24S and Hvo_0494C20S), and signal sequence deletion mutants (∆ss) of Hvo_1808. All proteins expressed had C-terminal Myc-tags except for Hvo_0494 and Hvo_0494C20S, which were C-terminally His-tagged. Myc and His-tagged proteins were detected using anti-Myc and anti-His antibodies, respectively. Comparable amounts of protein were loaded in each lane. The migration of molecular weight standards is indicated on the right. Predicted positions of precursor (p) and mature (m) proteins are indicated.
