Phosphorylation and Methylation of Proteasomal Proteins of the Haloarcheon Haloferax volcanii

Figure 2

Analysis of an H. volcanii psmA (𝛼1) knockout expressing wild type and variant α-type proteasomal genes in trans. (a) Growth and pigmentation of an H. volcanii psmA mutant (GZ130) were influenced by expression of α1 variant proteins on ATCC novobiocin plates. Synthesis of 𝛼1 Thr147Ala and 𝛼1 Thr158Ala inhibited growth of the psmA mutant, while 𝛼1 Ser58Ala and 𝛼1 Thr147Ala altered pigmentation. (b) No significant difference in 𝛼1 protein levels were detected in the H. volcanii psmA mutant synthesizing the wild type and variant 𝛼1 proteins from genes provided in trans. The α-type proteins encoded by the genes expressed in trans are indicated with the following abbreviations: wt, wild type; S58A, Ser58Ala; Y28F, Tyr28Phe; T158A, Thr158Ala; T147A, Thr147Ala.

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