S-Layer Glycoproteins and Flagellins: Reporters of Archaeal Posttranslational Modifications

Table 1

Effects of Hfx. volcanii agl deletions.

Gene	Role	Effect^1 of deletion on:
		Hfx. volcanii Growth in high salt	S-layer Assembly	Shedding	Susceptibility to protease	S-layer glycoprotein SDS-PAGE migration	N-linked glycan structure	Reference
aglB	OTase^2	Decreased	No effect	Increased	No effect	Increased	No glycan	[114, 123]
aglD	GTase^3 (sugar 5)	Decreased	Perturbed	Decreased	Decreased	Increased	Tetrasaccharide	[114, 123]
aglE	GTase (sugar 4)	No effect	n.d.	n.d.	No effect	No effect	Trisaccharide	[119]
aglF	Glucose-1-P uridyltransferase (sugar 3)	n.d.^4	n.d.	n.d.	Increased	Increased	Disaccharide	[120]
aglG	GTase (sugar 2)	n.d.	n.d.	n.d.	Increased	Increased	Monosaccharide	[120]
aglI	GTase (sugar 3)	n.d.	n.d.	n.d.	Increased	Increased	Disaccharide	[120]
aglM	UDP-glucose dehydrogenase (sugars 2, 3 (4?))	n.d.	n.d.	n.d.	Increased	Increased	Monosaccharide	[122]
aglP	Methyltransferase (sugar 4)	n.d.	n.d.	n.d.	Increased	n.d.	Modified tetrasaccharide	[124]

¹ Relative to level detected in parent strain; increased, decreased or no effect.
² OTase: oligosaccharyltransferase.
³ GTase: glycosyltransferase.
⁴ n.d.: not determined.