The Genome Sequence of Methanohalophilus mahii SLP^T Reveals Differences in the Energy Metabolism among Members of the Methanosarcinaceae Inhabiting Freshwater and Saline Environments

Table 3

The distribution of genes involved in the energy metabolism of members of the Methanosarcinaceae shows a correlation with the occupied habitat. The designation of genes is based on the proposed scheme of Rohlin and Gunsalus [42]. Phenotypic characteristics of the listed species were taken from Bergey’s Manual of Systematic Bacteriology [43]. Abbreviations: Msc, Methanosarcina; Mco, Methanococcoides; Msl, Methanosalsum; Mhb, Methanohalobium; Mhp, Methanohalophilus; +, genes present; −, genes not detected; (+), genes are present, but known to be silent. Note: at the time of writing, the genome sequence of Methanohalobium evestigatum (CP002069) was only available as draft.

	Msc.; barkeri	Msc.; mazei	Msc.; acetivorans	Mco.; burtonii	Msl.; zhilinae	Mhb.; evestigatum	Mhp.; mahii
Growth conditions:
Salinity of normal habitat	Freshwater	Freshwater	Marine	Marine	Hypersaline	Hypersaline	Hypersaline
NaCl requirement	−	−	+	+	+	+	+
NaCl optimum [M]	0.2	0.2	0.2	0.2	0.4– 0.7	4.3	1.2–2.0
Temp. optimum [°C]	35	35	37	23	45	50	35
Substrates:
Acetate	+	+	+	−	−	−	−
methanol, methylamines	+	+	+	+	+	+	+
H2+CO2	+	+	−	−	−	−	−
Presence of genes:
Strain sequenced	Fusaro	Gö1	C2A^T	ACE-MT	WeN5T	Z-7303T	SLPT
vhoGAC	−	+	−	−	−	−	−
vhtGACD	+	+	+	−		−	−
vhtGADF	−	−	−	−	+	−	−
vhtGAC	+	+	(+)	−	−	−	−
frhADGB	+	+	(+)	−	−	−	−
echABCDEF	+	+	−	−	−	−	−
fpoABCDHIJJKLMNO	+	+	+	+	+	+	+
rnfXCDGEABY	−	−	+	+	+	+	+
mrpABCDEFG	−	−	+	+	+	+	+
hdrED	+	+	+	+	+	+	+
hdrACB	+	+	+	+	+	+	+
ahaHIKECFABD	+	+	+	+	+	+	+