Improving the Catalytic Activity of Hyperthermophilic Pyrococcus horikoshii Prolidase for Detoxification of Organophosphorus Nerve Agents over a Broad Range of Temperatures

Figure 2

Relative activity with WT-Pfprol and P. horikoshii prolidase with OP nerve agent analog, p-nitrophenyl soman. All prolidase assays contained 50 mM MOPS 200 mM NaCl pH 7.0, 0.2 mM CoCl₂, and 3 mM p-nitrophenyl soman. One hundred percent relative activity corresponds to WT-Pfprol-specific activity for p-nitrophenyl soman of 0.26 μmol product formed/min/mg at 35°C, 0.33 μmol product formed/min/mg at 50°C, and 0.56 μmol product formed/min/mg at 70°C.