Characterization of Plasmid pPO1 from the Hyperacidophile Picrophilus oshimae

Table 1

Oligonucleotides used in this study.

Primer	Sequence (5′–3′)	Description
RT1.for/rev	AATATGCCCTGGAGATAGCG/AGACGACACTTTCCGATACG	Used in quantitative PCR for plasmid copy number determination
RT2.for/rev	ATGCACGAATGCCTACTCTC/GGGCAAAGGAAGGCTTAAAC
16S-Pt.for/rev	TCGTCCTTCCAGGATTACAG/CCGGCTTTGTAAATCTCCAG
RC1.rev1/2	TCGGATTTAACAGGGCGTCTA/CCTGAACTGCCTGTTATCAAT	ORF1 inner/outer 5′RACE
RC2.rev1/2	CAATAGGGTGGAGATGTTATAGC/TCAAAGCCCAGCCATTAGATTGC	ORF2 inner/outer 5′RACE
RC3.rev1/2	TTACCTGACCCTTTATTTCC/TCCAGGGCGTTTAAGTAAATG	ORF3 inner/outer 5′RACE
RC4.rev1/2	TTTCCCTGATATTCCCTTATCC/ACATCTCTTGGTATGCCTTTC	ORF4 inner/outer 5′RACE
RC5.rev1/2	TCGGAGTAGAATTTACCTGTAG/GGATAAGGATTACCTCTGTTAG	ORF5 inner/outer 5′RACE
RC6.rev1/2	GCATTTAGGACAGGTCGCATAC/ACTCACTACCACTTACATAC	ORF6 inner/outer 5′RACE