Sulfolobus Mutants, Generated via PCR Products, Which Lack Putative Enzymes of UV Photoproduct Repair

Table 2

Sensitivity of Saci_1096 mutant to DNA-damaging agents^a.

Damaging chemical	Type of damage	DG185 (con)	DG251 (con)	LR12
cisplatin (cis platinum(II) diammine dichloride)	Intra- and interstrand crosslinks	37.5 ± 0.0	37.5 ± 0.0	37.5 ± 0.0
MNNG (N-methyl N′-nitro N-nitrosoguanidine)	Base methylation	43.8 ± 28.6	43.8 ± 28.6	43.8 ± 28.6
Mechlorethamine (2-chloro-N-(2-chloroethyl)-N-methyl-ethanamine)	Intra- and interstrand crosslinks	250.0 ± 86.6	250.0 ± 86.6	250.0 ± 86.6
Metronidazole (2-(2-methyl-5-nitro-1H-imidazol-1-yl)ethanol)	Bulky adduct	2.5 × 10^3 ± 0	(no data)	2.5 × 10^3 ± 0
4-nitroqunoline N-oxide	Bulky adduct	0.4 ± 0.17	0.5 ± 0.17	0.5 ± 0.17
Butadiene diepoxide	Intrastrand crosslinks	100 ± 43.3	100 ± 43.3	100 ± 43.3
Hydrogen peroxide	Oxidation and strand breaks	93.8 ± 44.2	93.8 ± 44.2	93.8 ± 44.2

^a The average MIC values ± standard deviation (𝜇g/mL) were calculated from 3 replicates, except for hydrogen peroxide (average of two replicates).