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Archaea
Volume 2012 (2012), Article ID 873589, 10 pages
http://dx.doi.org/10.1155/2012/873589
Research Article

Identification of the Major Expressed S-Layer and Cell Surface-Layer-Related Proteins in the Model Methanogenic Archaea: Methanosarcina barkeri Fusaro and Methanosarcina acetivorans C2A

1Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, CA 90095, USA
2UCLA-DOE Institute for Genomics and Proteomics, University of California, Los Angeles, CA 90095, USA
3Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, USA
4Mass Spectrometry Resource, Boston University School of Medicine, 670 Albany Street, Rm 511, Boston, MA 02118, USA
5OPX Biotechnologies, Inc. Research Division, 2425 55th Street, Boulder, CO 80301, USA
6Department of Biological Chemistry, University of California, Los Angeles, CA 90095, USA

Received 28 December 2011; Accepted 2 February 2012

Academic Editor: Jerry Eichler

Copyright © 2012 Lars Rohlin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

A list the oligonucleotides used in this study is given (supplementary Table 1). The list of proteins identified by LC-MS/MS is given (supplementary Table 2) as are the peptides of Mbar_A17S8 detected by LC-MS/MS (supplementary Table 3). The abundances of transcripts from 68 genes annotated as cell surface or surface-related in the M. acetivorans genome are given (supplementary Table 4). A proposed RNA secondary structure for the UTR of Mbar A17S8 is given (supplementary Figure 1).

  1. Supplementary Material