Rings in the Extreme: PCNA Interactions and Adaptations in the Archaea
Figure 7
Adaptation to the high salt environment of HvoPCNA [20]. (a) Electrostatic surface representation of HvoPCNA (left—3IFV) and the more typical AfuPCNA (right—1RWZ), with the surface coloured from (red) to (blue), showing loss of the electropositive charge from the central pore of PCNA are calculated using APBS [30]. (b) The HvoPCNA binding pocket (top) shows a reduction in hydrophobic character and in depth of the binding pocket compared to AfuPCNA (bottom). The surfaces are coloured according to the Kyte-Doolittle scale with blue indicating the most hydrophilic residues through to orange for the most hydrophobic residues. The AfuFen1 peptide of 1RXZ is shown in magenta in both panels for comparison. Produced using Chimera [31].
