Microbial Diversity and Biochemical Potential Encoded by Thermal Spring Metagenomes Derived from the Kamchatka Peninsula

Table 2

Prokaryotic diversity and richness at 1, 3, and 20% genetic distance. Numbers of observed OTUs as well as Shannon and Chao1 indices were calculated with QIIME [16]. The maximal number of OTUs (𝑛_max) was calculated using the Michaelis-Menten-fit diversity metrics implemented in the QIIME package. Coverage was determined by dividing the observed number of OTUs with 𝑛_max.

Sample	Observed OTUs			Max. OTUs (𝑛max)			Coverage (%)			Shannon index (𝐻^′)			Chao1
	1%	3%	20%	1%	3%	20%	1%	3%	20%	1%	3%	20%	1%	3%	20%
	Archaea
Mutnovsky	33	25	7	37.7	27.4	7.2	88	91.2	97.2	2.96	2.70	1.87	44.3	28.3	7
Uzon	13	11	5	14	11.6	5	93	94.8	100	1.83	1.67	0.86	13	11	5
	Bacteria
Mutnovsky	17	12	10	18.2	12.3	10	93.4	97.6	100	2	1.81	1.65	32	15	13
Uzon	50	42	11	62.9	52.5	11.3	95.8	80.0	97.3	2.9	2.60	1.63	69.3	56.4	11.5