Microbial Diversity and Biochemical Potential Encoded by Thermal Spring Metagenomes Derived from the Kamchatka Peninsula

Table 4

Relative activities of recombinant esterases in the presence of different chemical compounds. The effect of the additives was further tested for significance. Significant effects (𝑃<0.05) are written in bold type.

Detergent	Relative activity of PlpBW1 (%)	Relative activity of EstBW1 (%)	Relative activity of EstBW2 (%)
AgNO3	106.80 ± 16.06	34.27 ± 1.66	163.17 ± 4.88
CaCl2	132.39 ± 9.63	85.35 ± 6.30	97.10 ± 0.98
CoCl2	131.17 ± 5.93	104.94 ± 7.29	64.00 ± 5.60
CTAB	274.39 ± 34.52	40.66 ± 6.67	8.11 ± 3.30
CuCl2	125.48 ± 2.70	81.03 ± 3.98	94.20 ± 0.57
EDTA	119.45 ± 7.50	101.47 ± 10.75	96.03 ± 4.24
FeCl3	106.67 ± 8.94	53.56 ± 12.56	99.47 ± 5.01
KCl	124.50 ± 19.51	99.29 ± 12.11	96.40 ± 5.55
2-Mercaptoethanol	84.82 ± 7.31	0.00 ± 0.00	78.74 ± 19.95
MgCl2	133.37 ± 7.25	90.38 ± 13.06	101.54 ± 2.87
MnCl2	72.07 ± 2.31	82.14 ± 14.16	48.90 ± 5.22
NaCl	131.24 ± 15.17	98.59 ± 20.07	92.42 ± 3.60
NiSO4	121.86 ± 14.71	89.64 ± 4.60	45.65 ± 8.03
PMSF	77.49 ± 16.91	0.08 ± 16.43	0 ± 4.16
SDS	68.45 ± 17.38	22.47 ± 9.64	39.49 ± 3.14
Tween 80	174.52 ± 2.29	60.70 ± 12.74	141.50 ± 4.94
ZnCl2	72.01 ± 1.77	59.26 ± 5.72	8.64 ± 1.71

Abbreviations: CTAB: cetyltrimethylammonium bromide; EDTA: ethylenediaminetetraacetic acid; SDS: sodium dodecyl sulfate; PMSF: phenylmethylsulfonyl fluoride.