Genetic Confirmation of the Role of Sulfopyruvate Decarboxylase in Coenzyme M Biosynthesis in Methanococcus maripaludis

Figure 4

Growth of the comE::Tn5 mutant strain S201 in response to varying concentrations of CoM. Cultures were grown in McNA after three transfers in medium without CoM. Grey, no added CoM; red, + 0.5 nM CoM; green, + 10 nM CoM; orange, + 250 nM CoM; dash black, + 1.4 μM CoM; black, + 146 μM CoM. Representative error bars indicate the standard deviation of three independent replicate cultures. The inoculum was ~1 × 10⁴ cells from the second transfer.