Archaea / 2013 / Article / Tab 3

Research Article

PH1: An Archaeovirus of Haloarcula hispanica Related to SH1 and HHIV-2

Table 3

Digestion of the PH1 genome by nucleasesa.

Nuclease (amount)Proteinase K-treatedUntreated
ControlPH1 DNAControlPH1 DNA

DNase I (RNase-free)
(100 U)
+b++b+
Exonuclease III
(100 U)
+b++b+
Mung bean nuclease
(10 U)
+c+c
Nuclease BAL-31
(1 U)
+b++b
RNase A
(5 μg/mL)
+d+d
T7 exonuclease
(10 U)
+b++b

Extracted nucleic acid from purified PH1 virus was treated with various nucleases and then analysed by gel electrophoresis to detect whether the nuclease digested (+) or did not digest (−) the genome. Control nucleic acids used to confirm activity of the nucleases were λ DNA, a DNA oligonucleotide, and yeast transfer RNA.