Archaeal Community Structures in the Solfataric Acidic Hot Springs with Different Temperatures and Elemental Compositions

Figure 2

Phylogenetic tree of archaeal 16S rRNA gene sequences detected in Kirishima hot springs. Bootstrap values (>50%) based on 1000 replicates are indicated at nodes. The scale bar indicates the number of nucleotide substitutions per position. Number in the parenthesis with phylotype name represents the number of clones of each phylotype. The DNA database accession numbers are also indicated in the parenthesis. Aquifex pyrophilus is used as an outgroup species. The phylotype names derived from Pond-A, Pond-B, Pond-C, and Pond-D shown in blue, yellow, red, and green, respectively. UTSCG: uncultured thermoacidic spring clone group [16], HWCG I: hot water crenarchaeotic group I [6, 46], HWCG II (known as UCIII): uncultured crenarchaeal group III [15, 45, 46], HWCG IV (also known as UCII) [45, 49, 50], DSAG (known as MBGB): deep-sea archaeal group (marine benthic group B) [49, 51], MCG: miscellaneous crenarchaeal group [5, 50, 52, 53], MG I: marine crenarchaeotic group I [49, 54], SAGMCG: South Africa gold mine group [52], SCG: soil crenarchaeotic group [52], UTRCG: uncultured Thaumarchaeota-related clone group [16], FSCG: forest soil crenarchaeotic group [6, 55], and MHVG-I: marine hydrothermal vent group I [15, 16, 52].