Localization of Methyl-Coenzyme M Reductase as Metabolic Marker for Diverse Methanogenic Archaea

Figure 3

Localization of MCR in Methanosarcina mazei (DSM 3647; a, b, c), Methanothermobacter marburgensis (d–g), and Methanothermobacter wolfei (h, i, j). On media without nickel depletion, the markers are localized in the cytoplasm (b, e, i). On nickel-depleted media with levulinic acid (c, f: 0.05 M; g 0.2 M; j: 0.1 M levulinic acid) a tendency to membrane localization is obvious (a, d, h are negative controls).

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