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Volume 2014 (2014), Article ID 374146, 11 pages
Research Article

Unique Characteristics of the Pyrrolysine System in the 7th Order of Methanogens: Implications for the Evolution of a Genetic Code Expansion Cassette

11EA-4678 CIDAM, Clermont Université, Université d’Auvergne, Place Henri Dunant, 63001 Clermont-Ferrand, France
2Department of Microbiology and Alimentary Pharmabiotic Centre, University College Cork, Western Road, Cork, Ireland
3Institut Pasteur, Department of Microbiology, Unité de Biologie Moléculaire du Gène chez les Extrêmophiles, 28 rue du Dr. Roux, 75015 Paris, France

Received 30 August 2013; Accepted 19 October 2013; Published 27 January 2014

Academic Editor: Kyung Mo Kim

Copyright © 2014 Guillaume Borrel et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Material: displays the individual phylogenies respectively of PylC, PylD, Pyl S and PylB (Figures S1 to S4). Briefly, phylogenies were inferred from maximum likelihood procedures: Datasets of protein homologues (strains and accession numbers on each figure) were aligned by MUSCLE [44] and unambiguously aligned positions were automatically selected by using the BMGE software [48] with a BLOSUM30 substitution matrix. Maximum likelihood trees were calculated by PhyML [49]. See the Materials and Methods section for more details.

  1. Supplementary Material