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Archaea
Volume 2014, Article ID 957145, 9 pages
http://dx.doi.org/10.1155/2014/957145
Research Article

Seasonal Effects in a Lake Sediment Archaeal Community of the Brazilian Savanna

1Department of Cell Biology, Biological Sciences Institute, University of Brasília, 70910-900 Brasília, DF, Brazil
2Department of Ecology, Biological Sciences Institute, University of Brasília, 70910-900 Brasília, DF, Brazil
3Department of Genomics Science and Biotechnology, Catholic University of Brasília, 70790-160 Brasília, DF, Brazil
4Embrapa-Agroenergy, 70770-901 Brasília, DF, Brazil

Received 23 April 2014; Revised 30 June 2014; Accepted 8 July 2014; Published 20 July 2014

Academic Editor: William B. Whitman

Copyright © 2014 Thiago Rodrigues et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The Cerrado is a biome that corresponds to 24% of Brazil’s territory. Only recently microbial communities of this biome have been investigated. Here we describe for the first time the diversity of archaeal communities from freshwater lake sediments of the Cerrado in the dry season and in the transition period between the dry and rainy seasons, when the first rains occur. Gene libraries were constructed, using Archaea-specific primers for the 16S rRNA and amoA genes. Analysis revealed marked differences between the archaeal communities found in the two seasons. I.1a and I.1c Thaumarchaeota were found in greater numbers in the transition period, while MCG Archaea was dominant on the dry season. Methanogens were only found in the dry season. Analysis of 16S rRNA sequences revealed lower diversity on the transition period. We detected archaeal amoA sequences in both seasons, but there were more OTUs during the dry season. These sequences were within the same cluster as Nitrosotalea devanaterra’s amoA gene. The principal coordinate analysis (PCoA) test revealed significant differences between samples from different seasons. These results provide information on archaeal diversity in freshwater lake sediments of the Cerrado and indicates that rain is likely a factor that impacts these communities.