Recombinant Cyclodextrinase from Thermococcus kodakarensis KOD1: Expression, Purification, and Enzymatic Characterization

Figure 1

Sequence and structure analysis of CDase-Tk. Cyclodextrinase sequences from T. kodakarensis KOD1 (CDase-Tk, Tk1770), Thermococcus sp. CL1 (CDase-Tc, YP_006424883.1), Thermococcus sp. B1001 (CDase-Tb, BAB18100.1), Pyrococcus furiosus (CDase-Pf, NP_579668.1), and Thermofilum pendens Hrk 5 (CDase-Tp, YP_920858.1) were aligned. The solid line indicates the four consensus regions conserved in the GH13 family. The asterisks show the positions of the three active sites. The conservation level of each residue is indicated by the height of the bars above each residue. The number at the ending of each line of amino acids indicates the number of the amino acid residues.