Recombinant Cyclodextrinase from Thermococcus kodakarensis KOD1: Expression, Purification, and Enzymatic Characterization

Figure 2

Purification of CDase-Tk. Supernatants of total proteins from recombinant E. coli were loaded on a DEAE column, and bound proteins were eluted by stepwise NaCl addition. Molecular mass standards are indicated at the left. Lane 1, crude protein extract from noninduced cells; lane 2, crude protein extract from IPTG-induced cells; lanes 3 and 4, proteins eluted by 50 mM NaCl from the DEAE column; lane 5, proteins eluted by 100 mM NaCl; lane 6, proteins eluted by 200 mM NaCl.