Research Article

Recombinant Cyclodextrinase from Thermococcus kodakarensis KOD1: Expression, Purification, and Enzymatic Characterization

Figure 5

Thin layer chromatography (TLC) of hydrolysis products from β-CD generated by CDase-Tk. Lane 1: 1% β-CD alone, Lanes 2 to 4: CDase-Tk which was reacted with substrates at 1% concentration at 65°C for 10, 30, or 60 min. Std indicates the oligosaccharide standard containing 1% glucose, maltotriose, maltopentaose, and maltoheptaose.