Isolation and Molecular Identification of Auxotrophic Mutants to Develop a Genetic Manipulation System for the Haloarchaeon Natrinema sp. J7-2

Figure 6

The possible integration position of exogenous DNA in the genome of transformants conferring amylase activity. Strain J7-2-1 was transformed with pUC19-HM1752-amyH and the schematic diagram shows the possible crossover event between the plasmid and J7-2-1 genome. (a) Plasmid pUC19-HM1752-amyH. (b) J7-2-1 genome with the mutated 1752 gene. (c) The possible integration position of exogenous DNA on the genome of the transformants conferring amylase activity.