Isolation and Molecular Identification of Auxotrophic Mutants to Develop a Genetic Manipulation System for the Haloarchaeon Natrinema sp. J7-2

Table 1

Strains, plasmids, and amplification fragments used in this work.

Strains, plasmids, and amplification fragments	Description	Source or reference
Strain
Natrinema sp. J7-2	Haloarchaeal strain isolated from a salt mine	CCTCC AB91141, China [24, 25, 27]
Natrinema sp. J7-2-1	Auxotrophic strain from J7-2, Leu^−	This work
Natrinema sp. J7-2-22	Auxotrophic strain from J7-2, Arg^−	This work
Natrinema sp. J7-2-26	Auxotrophic strain from J7-2, Lys^−	This work
Natrinema sp. J7-2-52	Auxotrophic strain from J7-2, Arg^−	This work
Escherichia coli DH5α	endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG 80dlacZM15 (lacZYA-argF)U169,  hsdR17(),	[42]
Escherichia coli JM110	rpsL thr leu thi lacY galK galT ara tonA tsx dam dcm glnV44 (lac-proAB) e14-[ traD36 lacI lacZM15] hsdR17()	[43]
Plasmid
pUC19	Cloning vector	Lab stock
pMD19T-simple	Sequence vector	Purchased from TaKaRa
pUC19-KM1752	pUC19 containing leuB gene with KpnI and MfeI from J7-2	This work
pUC19-HM1752	pUC19 containing the ORF of leuB gene with HindIII and MfeI from J7-2	This work
pUC19-HM4049	pUC19 containing dapD gene with HindIII and MfeI from J7-2	This work
pUC19-HM1752-amyH	pUC19-HM1752 containing amyH gene from Haloarcula hispanica DSM 4426	This work
pUC19-HM1752-bgaH	pUC19-HM1752 containing bgaH gene from Haloferax  alicantei	This work
Amplification fragments
KM1752	leuB gene containing promoter from J7-2 with KpnI and MfeI	This work
HM1752	Amplification fragments of leuB gene from J7-2 with HindIII and MfeI	This work
HM4049	Amplification fragments of dapD gene from J7-2 with HindIII and MfeI	This work